RNeasy Micro Kit

RNA isolation / purification Tissue - Human Tongue

Experiment
RNA isolation / purification Tissue - Human Tongue
Product
RNeasy Micro Kit from Qiagen
Manufacturer
Qiagen

Protocol tips

Upstream tips
- This kit can be used for the isolation of RNA from up to 5 mg of tissue.
Downstream tips
- Always blank the spectrophotometer with your elution buffer.

- When measuring 260 nm absorbance, a unit of 1 on this scale equals to 40 ng of RNA.

Publication protocol

DNA was digested with RNase free DNase (Qiagen Inc, Valencia, CA) followed by RNA cleanup with the RNeasy Micro kit (Qiagen). One microgram of RNA was reverse-transcribed with Superscript III (Invitrogen). Products were amplified for 35 cycles with gene-specific primers utilizing RedTaq Ready Mix (Sigma-Aldrich, Saint Louis, MO). “No cDNA” samples were prepared for each primer set. DNA contamination was tested with control, intron-spanning primers. Products were resolved in a 2% agarose gel.

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Reviews

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Discussion

Discussion

4 years ago

Author: Paul G. Macon United States

RNA isolation from tissue

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Discussion

5 years ago

Author: Aaron Stege Netherlands

Problem in phase separation after using serum/plasma kit

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

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Papers

Check out relevant papers found by Labettor's AI that are relevant for performing RNA isolation / purification Tissue - Human Tongue using RNeasy Micro Kit from Qiagen.

Paper title
Distribution of Y-Receptors in Murine Lingual Epithelia
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Manufacturer protocol

Download the product protocol from Qiagen for RNeasy Micro Kit below.

Download PDF Download manufacturer protocol

Videos

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